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Robboy SJ, Bentley RC, Russell P, Anderson MC, Mutter GL, Prat J.

Robboy’s Pathology of the Female Reproductive Tract.

Laboratory Testing A diagnosis of genital herpes infection should be confirmed by laboratory testing, since various causes of genital ulcers can have a similar clinical presentation.

There are three primary methods of testing: serological testing, viral culture, and nucleic acid-based testing (PCR).

In addition, its sensitivity, particularly for recurrent or healing lesions, can be as low as 25%.

An internal control is built into the assay to determine adequacy of the specimen.

PCR is more sensitive than culture, and more specific than serology for the detection and differentiation of clinically significant HSV-1 and HSV-2 infections.

Genital herpes virus (HSV) infection, the most frequent sexually transmitted disease worldwide, is estimated to affect over one million new American women each year (1).

People with genital HSV infection can develop recurrent and painful genital and perineal lesions with considerable associated psychosocial distress.

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